5F6E
Crystal Structure of human Ubc9 (K48A/K49A/E54A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2012-12-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.873, 35.537, 58.151 |
Unit cell angles | 90.00, 112.16, 90.00 |
Refinement procedure
Resolution | 22.407 - 1.120 |
R-factor | 0.1545 |
Rwork | 0.154 |
R-free | 0.16990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u9b |
RMSD bond length | 0.005 |
RMSD bond angle | 1.151 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.140 |
High resolution limit [Å] | 1.120 | 1.120 |
Rmerge | 0.040 | 0.319 |
Number of reflections | 67889 | |
<I/σ(I)> | 103.8 | 3.1 |
Completeness [%] | 91.4 | 51.7 |
Redundancy | 4.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 0.1M Tris pH 8.5, 8% (w/v) polyethylene glycol |