5F6D
Crystal structure of Ubc9 (K48A/K49A/E54A) complexed with Fragment 6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2013-11-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.734, 35.452, 57.979 |
Unit cell angles | 90.00, 111.63, 90.00 |
Refinement procedure
Resolution | 26.949 - 1.553 |
R-factor | 0.1725 |
Rwork | 0.171 |
R-free | 0.19620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u9b |
RMSD bond length | 0.006 |
RMSD bond angle | 1.122 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.041 | 0.255 |
Number of reflections | 26685 | |
<I/σ(I)> | 34.9 | 3.1 |
Completeness [%] | 95.3 | 70.9 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 294 | 0.1M Tris pH 8.5, 8% (w/v) polyethylene glycol 8000 |