5F5T
Crystal structure of the Prp38-MFAP1 complex of Chaetomium thermophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 195.550, 52.150, 104.600 |
| Unit cell angles | 90.00, 102.51, 90.00 |
Refinement procedure
| Resolution | 95.454 - 2.550 |
| R-factor | 0.1969 |
| Rwork | 0.196 |
| R-free | 0.22330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rz9 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.814 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.454 | 2.620 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 33726 | |
| <I/σ(I)> | 17.9 | 1.9 |
| Completeness [%] | 98.9 | 98.4 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 277.15 | 0.1 M Tris-HCl, pH 8.5, 0.2 M ammonium acetate, and 20 % (v/v) isopropanol |
