5F52
Erwinia chrysanthemi L-asparaginase + Aspartic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.957, 88.216, 176.075 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.037 - 1.630 |
| R-factor | 0.1574 |
| Rwork | 0.156 |
| R-free | 0.18720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o7j |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.830 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.037 | 1.730 |
| High resolution limit [Å] | 1.629 | 1.630 |
| Rmerge | 0.076 | |
| Number of reflections | 151132 | |
| <I/σ(I)> | 15.65 | 2.92 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 7.25 | 6.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285 | PEG MME 2000 |
