5F4T
Crystal structure of the human sperm Izumo1 residues 22-254
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-30 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97949 |
Spacegroup name | H 3 |
Unit cell lengths | 126.039, 126.039, 60.895 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.642 - 3.083 |
R-factor | 0.2209 |
Rwork | 0.219 |
R-free | 0.24840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f4e |
RMSD bond length | 0.004 |
RMSD bond angle | 0.938 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.8) |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 63.020 | 40.640 | 3.300 |
High resolution limit [Å] | 3.080 | 8.720 | 3.080 |
Rmerge | 0.115 | 0.052 | 0.474 |
Rpim | 0.080 | 0.036 | 0.343 |
Total number of observations | 18861 | 831 | 2981 |
Number of reflections | 6484 | ||
<I/σ(I)> | 9 | 15.9 | 3.1 |
Completeness [%] | 97.4 | 96 | 91.3 |
Redundancy | 2.9 | 2.9 | 2.7 |
CC(1/2) | 0.993 | 0.994 | 0.774 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 295 | 0.07 M sodium acetate (pH 4.6), 5.6% (w/v) PEG 4000 and 30% (v/v) glycerol |