5F4P

HIV-1 gp120 complex with BNM-III-170

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	180
Detector technology	PIXEL
Collection date	2015-04-17
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	67.378, 127.517, 192.945
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.236 - 2.600
R-factor	0.2754
Rwork	0.274
R-free	0.31050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tgr
RMSD bond length	0.006
RMSD bond angle	1.064
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.236
High resolution limit [Å]	2.500
Rmerge	0.092
Number of reflections	26041
<I/σ(I)>	12.1
Completeness [%]	99.2
Redundancy	6.29

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		290	PEG 1500, calcium magnesium chloride, imidazole