5F47

Crystal structure of an aminoglycoside acetyltransferase meta-AAC0020 from an uncultured soil metagenomic sample in complex with trehalose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-11-02
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97856
Spacegroup name	C 1 2 1
Unit cell lengths	140.001, 53.954, 46.023
Unit cell angles	90.00, 105.19, 90.00

Refinement procedure

Resolution	26.977 - 1.497
R-factor	0.1566
Rwork	0.156
R-free	0.17760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	apoenzyme
RMSD bond length	0.008
RMSD bond angle	1.139
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.530
High resolution limit [Å]	1.497	1.497
Rmerge	0.052
Number of reflections	51615
<I/σ(I)>	32.39
Completeness [%]	96.8	93.6
Redundancy	7.4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	20% PEG3350, 0.2 M calcium chloride, 10 mM neomycin, 2% trehalose, paratone