5F3J
Crystal structure of DBP in complex with inhibitory monoclonal antibody 2D10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 93 |
Detector technology | CMOS |
Collection date | 2015-02-25 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00482 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.940, 63.940, 108.880 |
Unit cell angles | 90.00, 100.09, 90.00 |
Refinement procedure
Resolution | 19.944 - 4.001 |
R-factor | 0.2686 |
Rwork | 0.267 |
R-free | 0.29970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nuv |
RMSD bond length | 0.004 |
RMSD bond angle | 0.678 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 4.100 |
High resolution limit [Å] | 4.000 | 4.000 |
Rmerge | 0.116 | 0.275 |
Number of reflections | 9271 | |
<I/σ(I)> | 6.74 | 3.29 |
Completeness [%] | 92.9 | 91 |
Redundancy | 2.73 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 1% w/v tryptone, 50 mM HEPES sodium salt pH 7.0, 12% w/v PEG 3,350 |