5F2C
Thermostable aldehyde dehydrogenase from Pyrobaculum sp. 1860 crystallized in microgravity (complex with NADP+)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 185.356, 208.296, 163.811 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.145 - 1.898 |
| R-factor | 0.1854 |
| Rwork | 0.184 |
| R-free | 0.21750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4h73 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.023 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.145 | 1.919 |
| High resolution limit [Å] | 1.898 | 1.898 |
| Rmerge | 0.067 | |
| Number of reflections | 493450 | |
| <I/σ(I)> | 23 | |
| Completeness [%] | 87.6 | 22 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | Crystallization details 50 mM HEPES pH 7.5; 50 mM NaCl; 100 mM Imidazole pH 6.5; 1.6M Sodium acetate trihydrate. The crystal was grown in a microgravity environment. |
