5F16
CTA-modified hen egg-white lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2014-08-22 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.111, 78.111, 37.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.233 - 1.200 |
R-factor | 0.211 |
Rwork | 0.209 |
R-free | 0.24840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f14 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.989 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.233 | 1.260 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.094 | 1.337 |
Number of reflections | 36732 | |
<I/σ(I)> | 14.9 | 1.6 |
Completeness [%] | 99.3 | 99.7 |
Redundancy | 12.9 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 2M sodium chloride, 12% (w/v) PEG 6000 |