5F0Q
Crystal structure of C-terminal domain of the human DNA primase large subunit with bound DNA template/RNA primer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-08-16 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.987 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 122.507, 126.002, 83.942 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.480 - 2.210 |
Rwork | 0.223 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rr2 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
Rmerge | 0.062 | 0.040 | 0.379 |
Rmeas | 0.068 | 0.044 | 0.449 |
Rpim | 0.027 | 0.017 | 0.235 |
Total number of observations | 165370 | ||
Number of reflections | 31361 | ||
<I/σ(I)> | 9.6 | 1.55 | |
Completeness [%] | 95.4 | 99.4 | 58.3 |
Redundancy | 5.3 | 6.2 | 2.7 |
CC(1/2) | 0.995 | 0.745 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 0.18 M lithium sulfate, 50 mM sodium citrate, 2 mM TCEP, 0.1 M sodium malonate, 0.1 M Hepes-NaOH (pH 7.0), 19.7% PEG 8,000 and 0.02 M magnesium chloride |