5F0M
Structure of retromer VPS26-VPS35 subunits bound to SNX3 and DMT1 (SeMet labeled)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9750 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 369.480, 74.910, 57.390 |
| Unit cell angles | 90.00, 97.64, 90.00 |
Refinement procedure
| Resolution | 56.890 - 3.100 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.25370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f0l |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.161 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 56.890 | 3.280 | |
| High resolution limit [Å] | 3.100 | 9.180 | 3.100 |
| Rmerge | 0.105 | 0.034 | 0.883 |
| Rmeas | 0.124 | 0.041 | 1.043 |
| Total number of observations | 195953 | ||
| Number of reflections | 55451 | 2131 | 8911 |
| <I/σ(I)> | 8.57 | 31.17 | 1.3 |
| Completeness [%] | 99.7 | 99.1 | 99.1 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.8 M AmSO4, 0.1 M MES pH 6.0, 5% Glycerol |
