5F0K

Structure of VPS35 N terminal region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979030
Spacegroup name	C 1 2 1
Unit cell lengths	200.700, 108.600, 167.900
Unit cell angles	90.00, 125.50, 90.00

Refinement procedure

Resolution	47.914 - 3.074
R-factor	0.2084
Rwork	0.206
R-free	0.25530
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.625
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.060
High resolution limit [Å]	2.950	6.350	2.950
Rmerge	0.063	0.029	0.656
Rmeas	0.081	0.038	0.841
Rpim	0.050	0.024	0.520
Total number of observations	141769
Number of reflections	62588
<I/σ(I)>	10.4
Completeness [%]	98.6	97.4	98.7
Redundancy	2.3	2.3	2.3
CC(1/2)		0.998	0.697

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	291	1.7 M AmSO4, 2% PEG 1000, 0.1 M Hepes pH 7.6