5F0E
Murine endoplasmic reticulum alpha-glucosidase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-20 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.96597 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 104.144, 173.773, 62.836 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.090 - 1.740 |
| R-factor | 0.143 |
| Rwork | 0.141 |
| R-free | 0.16900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l4z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.090 | 1.830 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 109959 | |
| <I/σ(I)> | 12.9 | 2.4 |
| Completeness [%] | 94.7 | 96 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 291 | 32% Morpheus ethylene glycol/PEG 8000 mix, 0.05 M Morpheus carbxylic acid mix, 0.1 M Morpheus buffer system 1 |
