5F07
Crystal structure of glutathione transferase F8 from Populus trichocarpa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979769 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.010, 55.290, 55.029 |
| Unit cell angles | 90.00, 119.89, 90.00 |
Refinement procedure
| Resolution | 39.017 - 1.500 |
| R-factor | 0.1654 |
| Rwork | 0.164 |
| R-free | 0.18810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ri6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.182 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.710 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.045 | 0.292 |
| Number of reflections | 37437 | |
| <I/σ(I)> | 14.9 | 3.5 |
| Completeness [%] | 99.6 | 98.4 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 277 | 25% PEG 4000, Na MES pH6.5, 0.2 M Mg chloride |
