5EZU
Crystal structure of the N-terminal domain of vaccinia virus immunomodulator A46 in complex with myristic acid.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-25 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.787, 59.580, 47.257 |
| Unit cell angles | 90.00, 117.70, 90.00 |
Refinement procedure
| Resolution | 41.842 - 1.550 |
| R-factor | 0.2017 |
| Rwork | 0.200 |
| R-free | 0.24180 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.025 |
| Data reduction software | XPREP |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX (dev_1938) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.840 | 1.650 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.036 | 0.540 |
| Total number of observations | 116682 | |
| Number of reflections | 23425 | |
| <I/σ(I)> | 12.8 | 1.53 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 1 | 1.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | PEG6000, sodium chloride, lithium chloride, ammonium chloride |
