5EZO

Crystal Structure of PfCyRPA in complex with an invasion-inhibitory antibody Fab.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-14
Detector	PSI PILATUS 6M
Wavelength(s)	1.000000
Spacegroup name	P 1 21 1
Unit cell lengths	95.801, 44.681, 121.336
Unit cell angles	90.00, 108.03, 90.00

Refinement procedure

Resolution	43.000 - 3.630
R-factor	0.2763
Rwork	0.274
R-free	0.33000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ezi 5ezn
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.000		3.720
High resolution limit [Å]	3.630	16.230	3.630
Rmerge	0.497	0.044	2.673
Rmeas	0.595	0.054	3.181
Total number of observations	37178
Number of reflections	11401	143	855
<I/σ(I)>	2.63	14.35	0.4
Completeness [%]	99.1	92.9	98.7
Redundancy	3.3		3.3
CC(1/2)	0.921	0.998	0.350

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.1 M sodium citrate pH 5.5, 17 % PEG 5000 MME, 0.2 M NDSB-201