5EZN

Crystal Structure of PfCyRPA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-05
Detector	PSI PILATUS 6M
Wavelength(s)	0.999980
Spacegroup name	P 1 21 1
Unit cell lengths	46.240, 78.500, 97.040
Unit cell angles	90.00, 94.85, 90.00

Refinement procedure

Resolution	43.000 - 2.510
R-factor	0.1866
Rwork	0.183
R-free	0.25010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ezo 5ezi
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.000		2.580
High resolution limit [Å]	2.510	11.230	2.510
Rmerge	0.046	0.014	1.631
Rmeas	0.055	0.017	1.934
Total number of observations	77219
Number of reflections	23448	277	1741
<I/σ(I)>	15.57	59.2	0.68
Completeness [%]	99.4	97.2	99.3
Redundancy	7.1		7.2
CC(1/2)	0.999	1.000	0.294

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0-0.5 M MgCl2, 20-25 % PEG 3350