5EYO
The crystal structure of the Max bHLH domain in complex with 5-carboxyl cytosine DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-20 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 32.750, 306.160, 49.060 |
| Unit cell angles | 90.00, 103.79, 90.00 |
Refinement procedure
| Resolution | 38.270 - 2.390 |
| R-factor | 0.2045 |
| Rwork | 0.203 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1an2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.546 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11_2558: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.270 | 2.450 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.067 | 0.953 |
| Number of reflections | 18442 | |
| <I/σ(I)> | 15.7 | 2 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 7.6 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 0.2 M Ammonium Sulfate, 0.1 M Sodium Citrate-HCl pH 5.6, 25% (w/v) PEG 4000 |
