5EY7
Crystal structure of Fructokinase from Vibrio cholerae O395 in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-11-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 107.116, 99.858, 61.548 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.409 - 2.461 |
| R-factor | 0.2169 |
| Rwork | 0.215 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tz6 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.737 |
| Data reduction software | AUTOMAR |
| Data scaling software | AUTOMAR |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.450 | 2.460 |
| Rmerge | 0.056 | 0.200 |
| Number of reflections | 23435 | |
| <I/σ(I)> | 6.7 | 1.9 |
| Completeness [%] | 94.3 | 95.01 |
| Redundancy | 2.73 | 2.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 5% PEG 6000, 0.1M MES, pH 6.0 |
