5EXU
Reversibly photoswitching protein Dathail, Ensemble refinement
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-10-17 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.250, 81.180, 39.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.180 - 1.650 |
| R-factor | 0.1711 |
| Rwork | 0.168 |
| R-free | 0.20990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.705 |
| Data reduction software | iMOSFLM |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.180 | 1.702 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.063 | 0.304 |
| Number of reflections | 29829 | |
| <I/σ(I)> | 10.91 | 2.18 |
| Completeness [%] | 98.6 | 88.1 |
| Redundancy | 5.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Magnesium Chloride, 0.1M Bis-Tris:HCl pH 6.5, 25% PEG 3350 |
