5EXR

Crystal structure of human primosome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	113.096, 210.164, 172.565
Unit cell angles	90.00, 93.56, 90.00

Refinement procedure

Resolution	39.940 - 3.600
Rwork	0.268
R-free	0.32600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qcl 4q5v 4rr2 4y97
RMSD bond length	0.012
RMSD bond angle	1.800
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.660
High resolution limit [Å]	3.600	3.600
Rmerge	0.063	0.364
Total number of observations	236349
Number of reflections	74238
<I/σ(I)>	12.2	1.93
Completeness [%]	80.5	72.7
Redundancy	2.6	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295	0.2 M lithium sulphate, 50 mM TRIS HCl pH 8.5, 2 mM TCEP pH 7.5, 11.2% w/v PEG 4,000, 3% v/v ethanol, 0.5% v/v polypropylene glycol P400 and 0.2 mM EDTA