5EXB

Wild type green fluorescent protein DendFP (Dendronephthya sp.)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	69.384, 106.397, 137.209
Unit cell angles	109.68, 100.01, 101.72

Refinement procedure

Resolution	37.370 - 1.810
R-factor	0.1828
Rwork	0.182
R-free	0.24930
RMSD bond length	0.021
RMSD bond angle	2.224
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.840
High resolution limit [Å]	1.810	4.910	1.810
Rmerge	0.033	0.016	0.244
Rmeas	0.046	0.023	0.345
Rpim	0.033	0.016	0.244
Total number of observations	615189
Number of reflections	310150
<I/σ(I)>	13.5
Completeness [%]	97.0	98.8	90.6
Redundancy	2	2	1.9
CC(1/2)		0.998	0.809

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Mg formate, 20% PEG 3350