5EX7

Crystal structure of Brat NHL domain in complex with an 8-nt hunchback mRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9788
Spacegroup name	P 32 2 1
Unit cell lengths	150.518, 150.518, 37.921
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.865 - 2.600
R-factor	0.1835
Rwork	0.181
R-free	0.23080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q7f
RMSD bond length	0.004
RMSD bond angle	0.737
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.082	0.049	0.652
Rmeas	0.086	0.052	0.685
Rpim	0.026	0.016	0.208
Total number of observations	169589
Number of reflections	15443
<I/σ(I)>	11.6
Completeness [%]	99.9	99.1	100
Redundancy	11	10.1	10.7
CC(1/2)		0.999	0.906

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	289	14% Jeffamine ED-2001, 0.1 M imidozale