5EWR
C merolae U4 snRNP protein Snu13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.12171 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.330, 57.582, 65.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.212 - 2.350 |
| R-factor | 0.169 |
| Rwork | 0.166 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ale |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.521 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.5) |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 6.370 | 2.350 |
| Rmerge | 0.110 | 0.160 | 0.251 |
| Rmeas | 0.114 | 0.167 | 0.260 |
| Rpim | 0.031 | 0.047 | 0.068 |
| Total number of observations | 70926 | ||
| Number of reflections | 5143 | ||
| <I/σ(I)> | 10.5 | ||
| Completeness [%] | 99.8 | 100 | 100 |
| Redundancy | 13.8 | 11.8 | 14.3 |
| CC(1/2) | 0.994 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 298 | 31% PEG 3350, 100 mM sodium acetate pH 4.4 |
