5EWR

C merolae U4 snRNP protein Snu13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.12171
Spacegroup name	P 21 21 21
Unit cell lengths	30.330, 57.582, 65.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.212 - 2.350
R-factor	0.169
Rwork	0.166
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ale
RMSD bond length	0.016
RMSD bond angle	1.521
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.5)
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.390
High resolution limit [Å]	2.350	6.370	2.350
Rmerge	0.110	0.160	0.251
Rmeas	0.114	0.167	0.260
Rpim	0.031	0.047	0.068
Total number of observations	70926
Number of reflections	5143
<I/σ(I)>	10.5
Completeness [%]	99.8	100	100
Redundancy	13.8	11.8	14.3
CC(1/2)		0.994	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	298	31% PEG 3350, 100 mM sodium acetate pH 4.4