5EWQ
The crystal structure of an amidase family protein from Bacillus anthracis str. Ames
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97937, 0.97951 |
| Spacegroup name | P 1 |
| Unit cell lengths | 77.390, 89.558, 89.600 |
| Unit cell angles | 112.03, 106.64, 106.55 |
Refinement procedure
| Resolution | 30.347 - 2.570 |
| R-factor | 0.2219 |
| Rwork | 0.220 |
| R-free | 0.25380 |
| Structure solution method | MAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.521 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.500 | 2.610 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.015 | 0.729 |
| Number of reflections | 59854 | |
| <I/σ(I)> | 27.3 | 1.3 |
| Completeness [%] | 97.9 | 97.8 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 24% (w/v) PEG1500, 20% (v/v) glycerol |
