5EW6

Structure of ligand binding region of uPARAP at pH 7.4 without calcium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	78.110, 78.110, 252.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.192 - 2.290
R-factor	0.1986
Rwork	0.197
R-free	0.23670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e4k
RMSD bond length	0.008
RMSD bond angle	1.120
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.610	2.350
High resolution limit [Å]	2.290	2.290
Rmerge	0.079	0.842
Number of reflections	36396
<I/σ(I)>	19.9	3
Completeness [%]	100.0	100
Redundancy	13.9	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	5-7% PEG 3350, 1 mM CaCl2, 100 mM HEPES, 10% PEG 3350, 5 mM EDTA