5EV4

Structure IV of Intact U2AF65 Recognizing the 3' Splice Site Signal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97945
Spacegroup name	P 21 21 21
Unit cell lengths	43.502, 63.411, 77.662
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.830 - 1.570
R-factor	0.1559
Rwork	0.154
R-free	0.17640
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.009
RMSD bond angle	1.054
Data reduction software	XDS
Data scaling software	Aimless (0.1.26)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.830	38.830	1.600
High resolution limit [Å]	1.570	8.600	1.570
Rmerge	0.022	0.014	0.149
Rpim	0.009	0.006	0.089
Total number of observations	186470	1268	3347
Number of reflections	30216
<I/σ(I)>	42.9	83.3	6.9
Completeness [%]	98.3	98.4	71.7
Redundancy	6.2	5.5	3.1
CC(1/2)	1.000	1.000	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	1M Succinic Acid, 0.1M HEPES, 3% PEG MME 2000