Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 204.557, 177.390, 244.553 |
| Unit cell angles | 90.00, 112.94, 90.00 |
Refinement procedure
| Resolution | 75.400 - 2.150 |
| R-factor | 0.1877 |
| Rwork | 0.186 |
| R-free | 0.21980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vbi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.307 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.470 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.160 | 0.610 |
| Number of reflections | 858032 | |
| <I/σ(I)> | 19.9 | 2.3 |
| Completeness [%] | 98.9 | 96.9 |
| Redundancy | 6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.15 M SODIUM CITRATE, PH 5.5, 14% PEG 3350, 1 MM PYRUVATE |
