5EU5
HLA Class I antigen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.330, 79.640, 57.740 |
Unit cell angles | 90.00, 116.22, 90.00 |
Refinement procedure
Resolution | 43.420 - 1.540 |
R-factor | 0.1715 |
Rwork | 0.170 |
R-free | 0.20060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i4w |
RMSD bond length | 0.018 |
RMSD bond angle | 1.914 |
Data reduction software | XDS |
Data scaling software | Aimless (0.2.12) |
Phasing software | PHASER (2.5.5) |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.420 | 43.420 | 1.580 |
High resolution limit [Å] | 1.540 | 6.890 | 1.540 |
Rmerge | 0.070 | 0.030 | 0.582 |
Rpim | 0.042 | 0.017 | 0.354 |
Total number of observations | 244577 | 2958 | 17745 |
Number of reflections | 67308 | ||
<I/σ(I)> | 13 | 35.9 | 2.3 |
Completeness [%] | 99.6 | 98.2 | 99.9 |
Redundancy | 3.6 | 3.8 | 3.6 |
CC(1/2) | 0.997 | 0.998 | 0.758 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1 M Hepes pH 7.5, 0.2 M Ammonium Sulphate, 25 % PEG 4000 |