5ETE
Structure of pathogen-related yeast protein, Pry1 in complex with a competitive inhibitor of cholesterol binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-04-14 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54056 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 124.740, 124.740, 59.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.485 - 2.100 |
| R-factor | 0.1682 |
| Rwork | 0.167 |
| R-free | 0.19040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1smb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.935 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2405)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.750 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 17583 |
| <I/σ(I)> | 15.4 |
| Completeness [%] | 99.8 |
| Redundancy | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 1.6M Ammonium sulphate, 0.1M MES pH 6.5, 10% v/v 1,4-dioxane |
