5ET9
Racemic crystal structures of Pribnow box consensus promoter sequence (P21/n)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21/n 1 |
| Unit cell lengths | 28.786, 68.288, 34.213 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 34.140 - 1.830 |
| R-factor | 0.2993 |
| Rwork | 0.298 |
| R-free | 0.32940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID: 1FQ2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.073 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.140 | 1.895 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.016 | 0.390 |
| Number of reflections | 10610 | |
| <I/σ(I)> | 17.5 | 1.87 |
| Completeness [%] | 92.2 | 65.75 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | DNA containing D- and L- sugars (racemic mixture), sodium cacodylate, barium chloride, potassium chloride, spermine tetrachloride, MPD |
