5ET6
Human muscle fructose-1,6-bisphosphatase in inactive T-state in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-08 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.82657 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 218.622, 234.733, 71.607 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.389 - 1.845 |
| R-factor | 0.1686 |
| Rwork | 0.168 |
| R-free | 0.19210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ifa |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.721 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.389 | 1.960 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.055 | 0.693 |
| Number of reflections | 156646 | |
| <I/σ(I)> | 19.43 | 2.99 |
| Completeness [%] | 99.2 | 97.4 |
| Redundancy | 5.04 | 4.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 1.6M ammonium citrate tribasic |
