5ET4
Structure of RNase A-K7H/R10H in complex with 3'-CMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-06 |
| Detector | MAR555 FLAT PANEL |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.366, 32.281, 106.963 |
| Unit cell angles | 90.00, 125.71, 90.00 |
Refinement procedure
| Resolution | 29.125 - 2.100 |
| R-factor | 0.2285 |
| Rwork | 0.224 |
| R-free | 0.32120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rpf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.212 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.710 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 26449 | |
| <I/σ(I)> | 10.7 | 3.2 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 800 uL crystallisation condition reservoir formed by 27% PEG4000 and 20 mM sodium cacodylate buffer, pH 5.0. Crystals grew from droplets of 1 uL of protein solution and an equal volume of reservoir solution. |
