5ET0

Crystal structure of Myo3b-ARB2 in complex with Espin1-AR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 1 2 1
Unit cell lengths	39.740, 68.780, 173.450
Unit cell angles	90.00, 90.04, 90.00

Refinement procedure

Resolution	34.690 - 2.300
R-factor	0.2247
Rwork	0.223
R-free	0.25340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5et1
RMSD bond length	0.010
RMSD bond angle	1.111
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	173.450	34.690	2.420
High resolution limit [Å]	2.300	7.270	2.300
Rmerge		0.041	0.799
Rmeas	0.120
Rpim	0.062	0.025	0.469
Total number of observations	148562	4445	22264
Number of reflections	39636
<I/σ(I)>	7.7	16.6	1.9
Completeness [%]	94.9	90.5	96.6
Redundancy	3.7	3.5	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	289	1.6 M Ammonium Sulfate, 0.1 M Tris