5ERS
GephE in complex with Mg(2+) - AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-23 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9640 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 87.002, 99.278, 112.791 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.639 - 1.700 |
| R-factor | 0.1547 |
| Rwork | 0.153 |
| R-free | 0.17850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pd0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.370 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.640 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.133 | 1.081 |
| Number of reflections | 53903 | |
| <I/σ(I)> | 9.2 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.5 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1 M sodium acetate, 34% MPD |
