5ERC

X-ray crystal structure of BRPF1 PZP domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-27
Detector	NOIR-1
Wavelength(s)	1.28
Spacegroup name	P 61 2 2
Unit cell lengths	73.870, 73.870, 147.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	63.973 - 2.050
R-factor	0.1651
Rwork	0.162
R-free	0.19340
RMSD bond length	0.007
RMSD bond angle	1.057
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	63.973
High resolution limit [Å]	2.050
Number of reflections	15595
<I/σ(I)>	19.6
Completeness [%]	100.0
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.2 M Calcium acetate, 0.1 M Imidazole pH 8.0, 10% PEG8000