5ER7

Connexin-26 Bound to Calcium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.000
Spacegroup name	H 3 2
Unit cell lengths	156.092, 156.092, 160.077
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.427 - 3.286
R-factor	0.2807
Rwork	0.279
R-free	0.31840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PolyAla build from a cryoEM map
RMSD bond length	0.004
RMSD bond angle	0.710
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.890
High resolution limit [Å]	2.840	7.690	2.840
Rmerge	0.077	0.036
Total number of observations	105396
Number of reflections	11481
<I/σ(I)>	5.4
Completeness [%]	99.8	98.8	98.9
Redundancy	5.8	5.3	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 M Hepes, 15 mM Sodium formate, 20 mM CaCl2, 14% (w/v) PEG 3350