5ER7
Connexin-26 Bound to Calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 156.092, 156.092, 160.077 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.427 - 3.286 |
| R-factor | 0.2807 |
| Rwork | 0.279 |
| R-free | 0.31840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PolyAla build from a cryoEM map |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.710 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.890 |
| High resolution limit [Å] | 2.840 | 7.690 | 2.840 |
| Rmerge | 0.077 | 0.036 | |
| Total number of observations | 105396 | ||
| Number of reflections | 11481 | ||
| <I/σ(I)> | 5.4 | ||
| Completeness [%] | 99.8 | 98.8 | 98.9 |
| Redundancy | 5.8 | 5.3 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1 M Hepes, 15 mM Sodium formate, 20 mM CaCl2, 14% (w/v) PEG 3350 |
