5EQO
Human Angiogenin in complex with sulphate anions at an acidic solution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0403 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 82.040, 37.450, 42.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.740 - 2.400 |
| R-factor | 0.18976 |
| Rwork | 0.186 |
| R-free | 0.25005 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5eop |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.900 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.138 | 0.401 |
| Number of reflections | 5550 | |
| <I/σ(I)> | 9.3 | 3.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 289 | 0.1 M sodium cacodylate pH 6.0 2.0 M ammonium sulphate |
