5EPZ
Human Angiogenin in complex with sulphate anions at a basic solution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-13 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0403 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 82.330, 37.420, 42.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.580 - 1.850 |
R-factor | 0.18483 |
Rwork | 0.183 |
R-free | 0.21749 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5eop |
RMSD bond length | 0.010 |
RMSD bond angle | 1.369 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.580 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.084 | 0.518 |
Number of reflections | 11802 | |
<I/σ(I)> | 22.4 | 6.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.7 | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 289 | 0.1 M Tris/HCl buffer pH 8.0, 2.4 M ammonium sulphate |