5EPV
Histidine kinase domain from the LOV-HK blue-light receptor from Brucella abortus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98011,1.80000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.390, 100.840, 71.410 |
| Unit cell angles | 90.00, 102.75, 90.00 |
Refinement procedure
| Resolution | 48.550 - 2.510 |
| R-factor | 0.2039 |
| Rwork | 0.202 |
| R-free | 0.24220 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.210 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.660 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmerge | 0.060 | 0.591 |
| Number of reflections | 32760 | |
| <I/σ(I)> | 14.3 | 2 |
| Completeness [%] | 97.7 | 95.1 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 295 | 7.5% w/v PEG8000, 0.1 M HEPES, pH 8.3 |
