5EPU
X-ray structure uridine phosphorylase from Vibrio cholerae in complex with cytosine at 1.06A.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97989 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.848, 96.715, 92.890 |
| Unit cell angles | 90.00, 119.96, 90.00 |
Refinement procedure
| Resolution | 46.460 - 1.060 |
| R-factor | 0.106 |
| Rwork | 0.105 |
| R-free | 0.12200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k6o |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.504 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.461 | 1.120 |
| High resolution limit [Å] | 1.060 | 1.060 |
| Rmerge | 0.066 | 0.608 |
| Number of reflections | 615831 | |
| <I/σ(I)> | 16.26 | 2.54 |
| Completeness [%] | 95.6 | 83.7 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG4000, 0.1M TRIS-HCl, 0.2M MgCl2x6H2O |
