5EPC

Crystal structure of wild-type human phosphoglucomutase 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2015-06-06
Detector	RDI CMOS_8M
Wavelength(s)	1.00001
Spacegroup name	P 41 21 2
Unit cell lengths	172.590, 172.590, 99.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.051 - 1.850
R-factor	0.171
Rwork	0.170
R-free	0.19750
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3pmg
RMSD bond length	0.007
RMSD bond angle	0.998
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.051	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.163	3.510
Number of reflections	150855
<I/σ(I)>	15.1	1
Completeness [%]	100.0	100
Redundancy	32.5	27.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Lithium or ammonium sulfate (1.35 - 1.85 M) with 0.1 M Tris, pH 8.0