5EP5
The crystal structure of the hypothetical protein SAV0944 mutant (Glu47Ala) from Staphylococcus aureus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.220, 91.570, 65.740 |
Unit cell angles | 90.00, 99.52, 90.00 |
Refinement procedure
Resolution | 46.000 - 2.900 |
R-factor | 0.21991 |
Rwork | 0.217 |
R-free | 0.26284 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.472 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 10339 |
<I/σ(I)> | 9.2 |
Completeness [%] | 89.7 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 1.5M Lithium sulphate, 0.1M HEPES Sodium pH7.5 |