5EP0

Quorum-Sensing Signal Integrator LuxO - Receiver+Catalytic Domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9782
Spacegroup name	P 61
Unit cell lengths	116.149, 116.149, 71.174
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.091 - 1.600
R-factor	0.1718
Rwork	0.171
R-free	0.19590
RMSD bond length	0.010
RMSD bond angle	1.053
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10-2155_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.048	0.026	0.651
Rmeas	0.052	0.028	0.700
Rpim	0.018	0.010	0.257
Total number of observations	563802
Number of reflections	72082
<I/σ(I)>	8.2
Completeness [%]	100.0	99.9	100
Redundancy	7.8	7.9	7.3
CC(1/2)		0.999	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	100 mM sodium buffer pH5.5, 2 M ammonium sulfate