5EOJ
Crystal structure of an antiparallel hexamer coiled-coil - ACC-Hex-PheI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.977 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 40.850, 40.850, 118.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.608 - 2.115 |
R-factor | 0.2456 |
Rwork | 0.244 |
R-free | 0.27530 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.638 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.610 | 2.191 |
High resolution limit [Å] | 2.115 | 2.115 |
Rmerge | 0.026 | 0.451 |
Number of reflections | 6246 | |
<I/σ(I)> | 13.42 | 1.12 |
Completeness [%] | 98.0 | 99 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 5 combined crystals from a range of crystal growing conditions: 0. 1 M Na formate ph 6.75 - 7.3 and PEG 3350 25-28% |