5EOC
Crystal structure of Fab C2 in complex with a Cyclic variant of Hepatitis C Virus E2 epitope I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-12 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.972, 56.191, 77.153 |
| Unit cell angles | 90.35, 90.18, 94.98 |
Refinement procedure
| Resolution | 15.000 - 1.980 |
| Rwork | 0.234 |
| R-free | 0.26565 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2Y5T AND 2Y6S |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.568 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.050 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.077 | |
| Number of reflections | 59746 | |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 93.0 | 81.7 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M ammonium sulphate and 25% (w/v) PEG 3,350 |
