5ENY
Ketosynthase from module 6 connected to acyl carrier protein from module 5 (unobservable) of the bacillaene synthase from Bacillus subtilis 168
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.106, 112.732, 211.441 |
| Unit cell angles | 104.96, 90.07, 106.32 |
Refinement procedure
| Resolution | 39.770 - 4.000 |
| R-factor | 0.33451 |
| Rwork | 0.333 |
| R-free | 0.35400 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.852 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.770 |
| High resolution limit [Å] | 4.000 |
| Number of reflections | 23712 |
| <I/σ(I)> | 6.1 |
| Completeness [%] | 98.7 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 25 mg/ml PksACP5+KS6, 150 mM NaCl, 10% glycerol, 10 mM HEPES, pH 7.5) with 1 uL crystallization buffer (30% PEG 4000 (v/v), 0.2 M sodium acetate, 0.1 M Tris, pH 8.5 |
