5EN8
Crystal structure of the N-terminal region of Smu1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.429, 35.233, 79.260 |
| Unit cell angles | 90.00, 93.27, 90.00 |
Refinement procedure
| Resolution | 40.049 - 2.230 |
| R-factor | 0.231 |
| Rwork | 0.229 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5en6 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.656 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.049 | 2.360 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Number of reflections | 21482 | |
| <I/σ(I)> | 11.1 | 1.1 |
| Completeness [%] | 95.9 | 89.1 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277.15 | 0.1 M MES, pH 6.5, 12 % [w/v] PEG 20,000 |
