5EML
Crystal structure of PRMT5:MEP50 with Compound 10 and SAM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-06-20 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.97872 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 103.400, 138.130, 178.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.370 - 2.390 |
R-factor | 0.212 |
Rwork | 0.208 |
R-free | 0.28210 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.413 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless (0.1.22) |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.060 | 42.060 | 2.450 |
High resolution limit [Å] | 2.390 | 10.690 | 2.390 |
Rmerge | 0.078 | 0.030 | 0.932 |
Rpim | 0.030 | 0.013 | 0.361 |
Total number of observations | 380569 | 4069 | 27992 |
Number of reflections | 50789 | ||
<I/σ(I)> | 19.4 | 58 | 2.8 |
Completeness [%] | 99.6 | 97.7 | 98.9 |
Redundancy | 7.5 | 6.4 | 7.6 |
CC(1/2) | 0.999 | 0.999 | 0.853 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Sodium acetate, 0.1 M Sodium citrate pH 6.1, 10% w/v PEG 4000 |